Andreas replied to the topic 'ASI Camera - Gain/Offset/x-Bit' in the forum. 3 years ago

I think this is a misconception. Calibration frames like bias or darks might be clipped on the left side of the histogram with offset 0. This could introduce artifacts during calibration.
scroll down to "Anatomy of Signals in Histograms". The offset value prevents the "separation" of beeing negative.