Hi Jason,
As Nou suggests it looks like you are moving the focuser too far from the focus point. So what value to set Initial Step Size to? There are a couple of ways:
1. Set it to the Critical Focus Zone of your Optical Train. The CFZ module will calculate it for your optical train but you'll need to supply 1 parameter which is how far in microns the focal plane moves when the focuser moves 1 tick. Its the highlighted field:
For refractors you can move the draw tube in and out, measure how far it moves with callipers / ruler and note down how many ticks the focuser moved. This will give you the number of microns / tick. For other telescopes where you are moving the primary or secondary mirror you'll need to investigate how to measure this with the telescope specs. If you are struggling with this if you send details of your telescope and focuser I'll see if I can figure it out from the online specs (some manufacturers publish the required info, but some don't unfortunately).
So in the screenshot, if you measured 1 tick to be equal to 1 micron then your CFZ would be 39 steps so try setting the Initial Step Size to that to start with. Once its working you can tweak to optimise it.
2. Start at focus and move the focuser (in or out it doesn't matter) a few ticks and take a frame. Keep repeating. What you are looking for is a step size that makes a difference to the HFR (displayed at the bottom of the v-curve) but doesn't go so far as to get bad values in the HFR. Once you get too far away from optimum focus star detection breaks down and the HFR will be incorrect. So you are after the sweet spot between moving enough to get different values for the HFR but not so far as to start to get star detection breaking down. When you have worked out a range from optimum focus to where star detection starts to break down then you have the operating range for Autofocus. I would suggest aiming to get around 10 datapoints so 5 either side of optimum focus. So divide your previously calculated range by 4 and that's your step size.
Backlash
You have 50 ticks in AF Overscan. Maybe that's fine. Can't tell. There's help in the manual for sorting out backlash if you're unsure. It will need to be sorted out in order for focus to work.
There are some other settings that you have that could probably be optimised but lets get the basic focus working before fiddling with those.